Membrane proteins are extracted from CAR-T cells and loaded on CARMA nanovesicles (CARMA-NVs)
Optimized lipid concentration and protein-to-lipid ratio to obtain nanovesicles with a mean diameter lower than 200 nm and a polydispersity index, PDI ~0.4
Lipid Nanovesicles without protein cargo showing a smooh surface
Confocal microscopy analysis of Rhodamine-labeled CARMA-NV uptake (red) in T-cells (nuclei in blue) after 48 hours of incubation.
Nanovesicles are engineered using synthetic lipids and CAR membrane proteins
Zeta potential analysis of CARMA NVs reveals a negative surface charge and good colloidal stability
Lipid Nanovesicles charged with CAR membrane proteins showing irregularities in the lipid bilayer consistent with protein payload
Time-lapse microscopy demonstrating the interaction of CARMA-NV treated T cells targeting tumor cell line.
